Intranasal peptide calculator

What this tool does

The intranasal calculator works out exactly how many sprays make up a dose for nasal peptide preparations such as BPC-157, Selank, Semax and NAD+. Every pump actuation releases a fixed volume — usually 0.1 mL — so once your concentration is known, the math is straightforward. Enter your preparation details and you'll get the mcg per spray, sprays required for your target dose, total actuations in the bottle and an estimated days of supply at your dosing frequency.

What it won't do is tell you what dose is right for you — it only handles the arithmetic. Dosing decisions belong with the research protocol you're following.

Why nasal delivery works

Spraying a peptide into the nose puts it in contact with the nasal mucosa — thin, heavily vascularized tissue with a large absorptive surface. Compounds can move into circulation quickly and skip digestion altogether, which is a problem for most peptides taken by mouth.

That said, not every peptide is a good nasal candidate. The ones that work best tend to share three traits: a molecular weight below roughly 6,000 Daltons so they can cross the mucosa, moderate lipophilicity to help membrane transport, and enough stability to stay active in an aqueous solution at nasal pH.

Expect roughly 5–20% bioavailability intranasally — well above the near-zero of oral dosing, but short of injection. That gap is exactly why nasal doses are usually set higher than injectable doses for the same compound: you're trading some efficiency for convenience.

Preparing a peptide nasal spray

1. 01

   Reconstitute the peptide

   Add chosen dilutant (saline water is best) to the vial. Run the stream down the inside wall and let the powder dissolve on its own — never shake it.

2. 02

   Work out your concentration

   Divide the total peptide (in mcg) by the diluent volume (in mL). Example: 5,000 mcg of BPC-157 in 2.5 mL yields 2,000 mcg/mL.

3. 03

   Move it into the spray bottle

   Draw the solution up with a syringe and transfer it into a clean nasal spray bottle. Standard pumps deliver about 0.1 mL per spray.

4. 04

   Prime before first use

   Pump a few times until a fine, even mist appears. Priming typically consumes 3–5 sprays, so factor that loss into your total dose count.

5. 05

   Count your doses

   Divide the bottle volume by 0.1 mL for total sprays, then divide by your sprays-per-dose to see how many doses the bottle holds.

For a full walkthrough of the math, see our injectable reconstitution calculator and peptide dosage protocol library.

Storage and shelf life

A reconstituted peptide degrades far faster than lyophilized powder, so how you store the bottle decides how long it stays potent.

• Keep it refrigerated. Store at 2–8°C, take it out only long enough to dose, and never freeze it.
• Block the light. Many peptides are photosensitive — an amber or opaque bottle is worth it.
• Plan on about 28–30 days. Most reconstituted peptides hold stability for roughly four weeks refrigerated. When unsure, mix smaller batches more often.
• Watch for degradation. Cloudiness, visible particles, a color shift or an off smell all mean discard and mix fresh.

Getting the most out of each spray

Technique matters — small habits change how much peptide actually reaches circulation.