Thymosin Alpha-1 dosage chart
10 mg Vial
Thymosin Alpha-1 is dosed at 300 mcg–500 mcg daily via subcutaneous injection in educational protocols. A 10 mg vial reconstituted with bacteriostatic water yields about 3.33 mg/mL. This information is for research and educational use only.
- Reconstitute
- Add 3.0 mL bacteriostatic water → ~3.33 mg/mL concentration.
- Typical daily range
- 300–500 mcg once daily (gradual titration).
- Easy measuring
- At 3.33 mg/mL, 1 unit = 0.01 mL ≈ 33.3 mcg on a U‑100 insulin syringe.
- Storage
- Lyophilized: refrigerate at 2–8 °C (35.6–46.4 °F) or freeze at −20 °C (−4 °F); after reconstitution, refrigerate and use within 7 days.
Dosing schedule
| Week | Daily Dose (mcg) | Units (per injection) (mL) |
|---|---|---|
| Week 1 | 300 mcg (0.3 mg) | 9 units (0.09 mL) |
| Weeks 2–8 | 500 mcg (0.5 mg) | 15 units (0.15 mL) |
Reconstitution steps
- 1Draw 3.0 mL bacteriostatic water with a sterile syringe.
- 2Inject slowly down the vial wall; avoid foaming.
- 3Gently swirl/roll until dissolved (do not shake).
- 4Label with reconstitution date and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.
Important Notes
Practical considerations for consistency and safety.
- Use new sterile insulin syringes; dispose in a sharps container.
- Rotate injection sites (abdomen, thighs, upper arms) to reduce local irritation.
- Inject slowly; wait a few seconds before withdrawing the needle.
- Document daily dose and site rotation to maintain consistency.
- Inspect solution before each use—do not use if cloudy or discolored.
How This Works
Thymosin Alpha-1 is a naturally occurring thymic peptide that modulates immune function through multiple pathways. It enhances the maturation and differentiation of T‑cells, augments dendritic cell function, and promotes the production of key cytokines including interferon‑α and interleukin‑2. Clinical studies have demonstrated Tα1’s ability to enhance immune responses in immunocompromised individuals, including those with chronic hepatitis B and C infections. Meta-analyses have also shown benefit in reducing mortality in moderate-to-critical COVID-19 patients.
Potential Benefits & Side Effects
Observations from preclinical and clinical literature.
- Supports enhanced T‑cell function and overall immune competence.
- Demonstrates an excellent safety profile; doses up to 1.6 mg twice weekly for 6–12 months have been well-tolerated.
- Even at experimental doses up to 16 mg SC over 12 months, no significant Tα1‑specific toxicity has been observed.
- Most common adverse effect is mild injection‑site irritation (redness or discomfort).
Lifestyle Factors
Complementary strategies for best outcomes.
- Maintain adequate sleep and stress management to support immune function.
- Consume a nutrient‑dense diet rich in vitamins C, D, and zinc.
- Engage in moderate physical activity to complement immune optimization.
- Avoid excessive alcohol and smoking, which impair immune responses.
Injection Technique
General subcutaneous guidance from clinical best‑practice resources.
- Clean the vial stopper and skin with alcohol; allow to dry.
- Pinch a skinfold; insert the needle at 45–90° into subcutaneous tissue.
- Do not aspirate for subcutaneous injections; inject slowly and steadily.
- Rotate sites systematically (abdomen, thighs, upper arms) to avoid lipohypertrophy.
important-note
This content is intended for therapeutic educational purposes only and does not constitute medical advice, diagnosis, or treatment.
Thymosin Alpha-1 dosage FAQs
How much bacteriostatic water do you use to reconstitute Thymosin Alpha-1?
Add 3.0 mL bacteriostatic water → ~3.33 mg/mL concentration. for a 10 mg Vial vial. Add the water slowly against the inside wall of the vial and swirl — never shake — until the powder fully dissolves.
What does the Thymosin Alpha-1 dosing schedule look like?
Week 1 — 300 mcg (0.3 mg) — 9 units (0.09 mL); Weeks 2–8 — 500 mcg (0.5 mg) — 15 units (0.15 mL). The full table above lists every step with matching syringe units.
What are the mixing steps for Thymosin Alpha-1?
Draw 3.0 mL bacteriostatic water with a sterile syringe. Inject slowly down the vial wall; avoid foaming. Gently swirl/roll until dissolved (do not shake).
How should reconstituted Thymosin Alpha-1 be stored?
Storage: Lyophilized: refrigerate at 2–8 °C (35.6–46.4 °F) or freeze at −20 °C (−4 °F); after reconstitution, refrigerate and use within 7 days..
How many insulin syringe units equal a Thymosin Alpha-1 dose?
Units depend on the water volume you used. Divide your target dose by the vial concentration to get millilitres, then multiply by 100 — 1 unit on a U-100 insulin syringe is 0.01 mL. The Spartan Peptides reconstitution calculator does this conversion for you.
Is Thymosin Alpha-1 approved for human use?
No. Thymosin Alpha-1 is sold and referenced strictly as a research chemical. Nothing on this page is medical advice and the material is not for human consumption.
Educational and research information only. Not medical advice, not for human consumption. Values are reference figures — always verify your own math with the reconstitution calculator.
On any insulin syringe, 1 unit = 0.01 mL, so unit counts above hold across brands.

