Tesamorelin + Ipamorelin dosage chart

10 mg Blend

Tesamorelin is dosed at 125 mcg–1 mg daily by subcutaneous injection in educational protocols, typically at night. A 10mg blend reconstituted with bacteriostatic water yields about 3.33 mg/mL. This information is for research and educational use only.

Reconstitute: Add 3.0 mL bacteriostatic water to the 10 mg vial → ~3.33 mg/mL total (1.67 mg/mL each peptide).
Typical daily range: 250–2000 mcg tesamorelin + 125–1000 mcg ipamorelin (gradual titration).
Easy measuring: At 3.33 mg/mL total, 1 unit = 0.01 mL ≈ 16.7 mcg of each peptide on a U‑100 insulin syringe.
Storage: Lyophilized: refrigerate at 2–8 °C (35.6–46.4 °F); after reconstitution, use immediately or refrigerate and use within 24–48 hours.
Injection frequency: Once daily — 5 days on, 2 days off (dose Mon–Fri, rest Sat–Sun).
Dosing schedule: 5 days on / 2 days off: dose Monday through Friday, then take Saturday and Sunday off. That is 5 dosing days per week.

Standard / Gradual Approach (3 mL = ~3.33 mg/mL total)

WEEK	TESAMORELIN DOSE	IPAMORELIN DOSE	UNITS (ML)
Weeks 1–2	250 mcg (0.25 mg)	125 mcg (0.125 mg)	23 units (0.23 mL)
Weeks 3–4	500 mcg (0.5 mg)	250 mcg (0.25 mg)	45 units (0.45 mL)
Weeks 5–6	1000 mcg (1.0 mg)	500 mcg (0.5 mg)	90 units (0.90 mL)
Weeks 7–10	1500 mcg (1.5 mg)	750 mcg (0.75 mg)	135 units (1.35 mL)
Weeks 11–16	2000 mcg (2.0 mg)	1000 mcg (1.0 mg)	180 units (1.80 mL)

Reconstitution steps

1Draw 3.0 mL bacteriostatic water with a sterile syringe.
2Inject slowly down the vial wall; avoid foaming.
3Gently swirl/roll until dissolved (do not shake).
4Label and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light; use within 24–48 hours.

Dosing Cycle & Timing

This protocol runs on a 5 days on / 2 days off cycle.

Cycle: 5 days on, 2 days off — dose Monday through Friday and rest on the weekend.
5 dosing days per week; the 2 off days help limit receptor desensitisation and give injection sites time to recover.
Keep the dose time consistent on dosing days.
If a weekday dose is missed, take it the same day if possible — do not dose on an off day to catch up.
Weekly totals in the supplies and shopping lists are calculated on 5 dosing days, not 7.

Protocol Overview

Concise summary of the once‑daily regimen.

Goal: Support pulsatile GH release through dual GHRH + ghrelin pathway stimulation.
Schedule: Daily subcutaneous injections for 8–16 weeks.
Dose Range: Tesamorelin 250–2000 mcg + ipamorelin 125–1000 mcg daily with gradual titration.
Reconstitution: 3.0 mL per 10 mg vial (~3.33 mg/mL total) for accurate unit measurements.
Storage: Lyophilized refrigerated 2–8 °C; reconstituted use immediately or within 24–48 hours.

Dosing Protocol

Suggested daily titration approach.

Start: 250 mcg tesamorelin + 125 mcg ipamorelin daily; increase every 2 weeks.
Target: 1500–2000 mcg tesamorelin + 750–1000 mcg ipamorelin by Weeks 7–16.
Frequency: Once per day (subcutaneous).
Cycle Length: 8–16 weeks.
Timing: Evening or bedtime administration may align with natural GH secretion patterns; rotate injection sites.

Storage Instructions

Proper storage preserves peptide quality.

Lyophilized: Store at 2–8 °C (35.6–46.4 °F) in dry, dark conditions; may be kept at ≤25 °C (77 °F) up to 3 months after dispensing.
Reconstituted: Inject immediately after reconstitution; if stored, refrigerate and use within 24–48 hours; do not freeze.
Allow vials to reach room temperature before opening to reduce condensation uptake.

Important Notes

Practical considerations for consistency and safety.

Use new sterile insulin syringes; dispose in a sharps container.
Rotate injection sites (abdomen, thighs, upper arms) to reduce local irritation.
Inject slowly; wait a few seconds before withdrawing the needle.
Document daily dose and site rotation to maintain consistency.
Higher‑volume injections (≥1.0 mL) may be split between two sites for comfort.

How This Works

Tesamorelin is a synthetic 44‑amino‑acid GHRH analog that stimulates pituitary GH release. Ipamorelin is a pentapeptide ghrelin mimetic that acts on the GHS‑R1a receptor, selectively stimulating GH secretion without significantly affecting cortisol, prolactin, or ACTH. Combined GHRH + GHRP/ghrelin‑mimetic administration produces synergistic, amplified GH pulses compared to either agent alone. Human pharmacokinetic studies show ipamorelin achieves peak GH release approximately 40 minutes post‑dose.

Potential Benefits & Side Effects

Observations from preclinical and clinical literature.

Enhanced pulsatile GH secretion through dual‑pathway stimulation.
Tesamorelin (2 mg/day) demonstrated reductions in trunk fat and visceral adipose tissue in HIV‑associated lipodystrophy trials.
Ipamorelin shows high GH selectivity with minimal impact on cortisol or other hormones.
Common adverse events with tesamorelin include injection‑site reactions (erythema, pruritus), arthralgia, and peripheral edema.
Ipamorelin is generally well tolerated; transient flushing or headache may occur.

Lifestyle Factors

Complementary strategies for best outcomes.

Pair with a balanced, protein‑forward diet tailored to energy needs.
Combine resistance training and aerobic activity to reinforce metabolic adaptations.
Prioritize sleep and stress management to support adherence and recovery.
Evening dosing may align with natural nocturnal GH secretion patterns.

Injection Technique

General subcutaneous guidance from clinical best‑practice resources.

Clean the vial stopper and skin with alcohol; allow to dry.
Pinch a skinfold; insert the needle at 45–90° into subcutaneous tissue.
Do not aspirate for subcutaneous injections; inject slowly and steadily.
Rotate sites systematically (abdomen, thighs, upper arms) to avoid lipohypertrophy.

Important Note

This content is for educational purposes only and is not medical advice.

Supplies needed

Plan covers the full 16-week protocol at the lowest listed starting dose: 0.77 mg per dose, 5 days on / 2 days off (80 administrations in total), using 10 mg vials. After mixing with 3 mL, the concentration is 3.33 mg/mL and each dose requires 0.23 mL total.

Tesamorelin + Ipamorelin vials (10 mg Blend each)

Vials are rounded up only after calculating the whole protocol: ceil(61.36 ÷ 10) = 7. Usable peptide remaining in an opened vial carries forward to the next dose.

Full protocol (16 weeks): 7 vials (61.36 mg needed ÷ 10 mg)

Insulin syringes (U-100, 1 mL)

One syringe per draw — some doses need more than one draw (dose exceeds a single 1 mL syringe or finishes a vial).

Full protocol (16 weeks): 86 syringes

Bacteriostatic water (10 mL bottles)

Use 3 mL per vial for reconstitution (7 × 3 mL = 21 mL).

Full protocol (16 weeks): 3 × 10 mL bottles

Alcohol swabs

One for the vial stopper per draw + one for each injection site (5 days on / 2 days off). Recommend 2 × 100-count boxes.

Full protocol (16 weeks): 166 swabs

Supply	Full protocol (16 weeks)
Tesamorelin + Ipamorelin vials (10 mg Blend each)Vials are rounded up only after calculating the whole protocol: ceil(61.36 ÷ 10) = 7. Usable peptide remaining in an opened vial carries forward to the next dose.	7 vials (61.36 mg needed ÷ 10 mg)
Insulin syringes (U-100, 1 mL)One syringe per draw — some doses need more than one draw (dose exceeds a single 1 mL syringe or finishes a vial).	86 syringes
Bacteriostatic water (10 mL bottles)Use 3 mL per vial for reconstitution (7 × 3 mL = 21 mL).	3 × 10 mL bottles
Alcohol swabsOne for the vial stopper per draw + one for each injection site (5 days on / 2 days off). Recommend 2 × 100-count boxes.	166 swabs

Tesamorelin + Ipamorelin dosage FAQs

How much bacteriostatic water do you use to reconstitute Tesamorelin + Ipamorelin?

Add 3.0 mL bacteriostatic water to the 10 mg vial → ~3.33 mg/mL total (1.67 mg/mL each peptide). for a 10 mg Blend vial. Add the water slowly against the inside wall of the vial and swirl — never shake — until the powder fully dissolves.

How often is Tesamorelin + Ipamorelin administered in research protocols?

Injection frequency: Once daily — 5 days on, 2 days off (dose Mon–Fri, rest Sat–Sun).

What does the Tesamorelin + Ipamorelin standard / gradual approach (3 ml = ~3.33 mg/ml total) look like?

Weeks 1–2 — 250 mcg (0.25 mg) — 125 mcg (0.125 mg) — 23 units (0.23 mL); Weeks 3–4 — 500 mcg (0.5 mg) — 250 mcg (0.25 mg) — 45 units (0.45 mL); Weeks 5–6 — 1000 mcg (1.0 mg) — 500 mcg (0.5 mg) — 90 units (0.90 mL); Weeks 7–10 — 1500 mcg (1.5 mg) — 750 mcg (0.75 mg) — 135 units (1.35 mL). The full table above lists every step with matching syringe units.

What are the mixing steps for Tesamorelin + Ipamorelin?

Draw 3.0 mL bacteriostatic water with a sterile syringe. Inject slowly down the vial wall; avoid foaming. Gently swirl/roll until dissolved (do not shake).

How should reconstituted Tesamorelin + Ipamorelin be stored?

Storage: Lyophilized: refrigerate at 2–8 °C (35.6–46.4 °F); after reconstitution, use immediately or refrigerate and use within 24–48 hours..

How many insulin syringe units equal a Tesamorelin + Ipamorelin dose?

Units depend on the water volume you used. Divide your target dose by the vial concentration to get millilitres, then multiply by 100 — 1 unit on a U-100 insulin syringe is 0.01 mL. The Spartan Peptides reconstitution calculator does this conversion for you.

Is Tesamorelin + Ipamorelin approved for human use?

No. Tesamorelin + Ipamorelin is sold and referenced strictly as a research chemical. Nothing on this page is medical advice and the material is not for human consumption.

Educational and research information only. Not medical advice, not for human consumption. Values are reference figures — always verify your own math with the reconstitution calculator.

On any insulin syringe, 1 unit = 0.01 mL, so unit counts above hold across brands.