Spartan Peptides LLC
All dosage protocols

TB-500 dosage chart

10 mg Vial

TB-500 (Thymosin Beta-4 Fragment 500) is a synthetic peptide known for its potential to support tissue repair, promote healing, reduce inflammation, and enhance recovery. Spartan Peptides protocol: mix 10mg TB-500 with 1mL BAC water.

Mixing guide
Mix 10MG TB-500 with 1mL BAC water.
Concentration
10 mg/mL (0.1mg per unit)
Recommended dosage
2mg – 5mg per dose
Injection frequency
Typically researched 2x weekly
Syringe
Use an insulin syringe (U-100) for accurate dosing. 1 unit = 0.01 mL.
Storage
Store reconstituted vial refrigerated at 2–8 °C, protected from light. Do not freeze.

TB-500 dosing guide (10mg / 1mL BAC water)

Units (insulin syringe)Amount (mg)Recommended dosage
20 units2mg2mg – 5mg
30 units3mg
40 units4mg
50 units5mg

Reconstitution steps

  1. 1Draw 1mL of bacteriostatic water into a sterile syringe.
  2. 2Inject the water slowly down the inside wall of the 10MG TB-500 vial.
  3. 3Swirl gently until fully dissolved — do not shake.
  4. 4Use a U-100 insulin syringe for accurate dosing.
  5. 5Store reconstituted solution refrigerated at 2–8 °C, protected from light.

Spartan Peptides official protocol

10mg total / 1mL = 0.1mg per unit. Use only within the 2–5mg range. Common research range: 2mg–5mg per dose, 2 times per week.

  • Mixing guide: mix 10MG TB-500 with 1mL BAC water.
  • Concentration after mixing: 10 mg/mL (0.1mg per unit).
  • Recommended dosage: 2mg – 5mg per dose.
  • Injection frequency: Typically researched 2x weekly.
  • Use an insulin syringe (U-100) for accurate dosing.

Reported research focus

Research purposes only. Premium quality. Built on legacy. Backed by science.

  • Supports tissue repair*
  • Reduces inflammation*
  • Enhances recovery*
  • Supports overall wellness*

Important Notes

Practical considerations for consistency and safety in research protocols.

  • Use new sterile insulin syringes for each injection; dispose in a sharps container.
  • Rotate injection sites (abdomen, thighs, upper arms) to reduce local irritation and lipohypertrophy.
  • Inject slowly; wait a few seconds before withdrawing the needle to prevent backflow.
  • Document daily dose, injection site, and any observations to maintain consistency.
  • Regulatory Note: TB‑500 is banned by WADA for athletic use and is not FDA‑approved for human administration.

How This Works

TB‑500 represents the N‑terminal active fragment of thymosin beta‑4, specifically the heptapeptide sequence Ac‑LKKTETQ. This region is responsible for the actin‑binding and cell‑migration properties of the full thymosin molecule. Preclinical studies demonstrate that TB‑500 promotes angiogenesis, accelerates wound healing, and supports tissue regeneration by upregulating cell motility and blood vessel formation. Research in animal models shows enhanced collagen deposition and reduced healing time in injury sites treated with thymosin fragments. Recent metabolic studies suggest TB‑500 may act as a prodrug, cleaving to an active pentapeptide metabolite that drives biological activity.

Potential Benefits & Side Effects

Observations from preclinical and veterinary literature.

  • Supports accelerated wound healing and tissue repair through enhanced angiogenesis and cell migration.
  • May reduce inflammation and fibrosis indirectly via thymosin pathways observed in animal models.
  • Generally well tolerated in veterinary studies; occasional mild injection‑site reactions (redness, tenderness) reported.
  • Human safety data is limited; no large‑scale clinical trials have been completed for TB‑500 specifically.

Lifestyle Factors

Complementary strategies for optimal research outcomes.

  • Maintain adequate protein intake to support tissue repair and regeneration processes.
  • Combine with appropriate physical activity; avoid overtraining during injury recovery phases.
  • Prioritize sleep (7–9 hours) to maximize natural recovery and repair mechanisms.
  • Manage stress levels through evidence‑based practices to support overall healing.

Injection Technique

General subcutaneous guidance from clinical best‑practice resources.

  • Clean the vial stopper and skin with alcohol; allow to air dry completely.
  • Pinch a skinfold at the injection site; insert the needle at 45–90° into subcutaneous tissue.
  • Do not aspirate for subcutaneous injections; inject slowly and steadily.
  • Rotate sites systematically within approved areas (abdomen, thighs, upper arms) to avoid lipohypertrophy.
  • Wait 5–10 seconds after injection before withdrawing needle to prevent medication leakage.

important-note

This content is intended for therapeutic educational purposes only and does not constitute medical advice, diagnosis, or treatment. TB‑500 is not approved by the FDA for human use and is for research purposes only. TB‑500 is a prohibited substance in competitive sports under WADA regulations.

TB-500 dosage FAQs

How do you reconstitute a 10 mg Vial vial of TB-500?

Add bacteriostatic water slowly down the inside wall of the 10 mg Vial TB-500 vial, swirl gently until clear, and use the reconstitution calculator to convert your chosen water volume into syringe units.

What is a typical TB-500 dose?

Recommended dosage: 2mg – 5mg per dose. Figures are research reference values only.

How often is TB-500 administered in research protocols?

Injection frequency: Typically researched 2x weekly.

What concentration does reconstituted TB-500 end up at?

Concentration: 10 mg/mL (0.1mg per unit). On any insulin syringe 1 unit = 0.01 mL, so unit counts convert directly across brands.

What does the TB-500 tb-500 dosing guide (10mg / 1ml bac water) look like?

20 units — 2mg — 2mg – 5mg; 30 units — 3mg; 40 units — 4mg; 50 units — 5mg. The full table above lists every step with matching syringe units.

What are the mixing steps for TB-500?

Draw 1mL of bacteriostatic water into a sterile syringe. Inject the water slowly down the inside wall of the 10MG TB-500 vial. Swirl gently until fully dissolved — do not shake.

How should reconstituted TB-500 be stored?

Storage: Store reconstituted vial refrigerated at 2–8 °C, protected from light. Do not freeze..

How many insulin syringe units equal a TB-500 dose?

Units depend on the water volume you used. Divide your target dose by the vial concentration to get millilitres, then multiply by 100 — 1 unit on a U-100 insulin syringe is 0.01 mL. The Spartan Peptides reconstitution calculator does this conversion for you.

Is TB-500 approved for human use?

No. TB-500 is sold and referenced strictly as a research chemical. Nothing on this page is medical advice and the material is not for human consumption.

Educational and research information only. Not medical advice, not for human consumption. Values are reference figures — always verify your own math with the reconstitution calculator.

On any insulin syringe, 1 unit = 0.01 mL, so unit counts above hold across brands.