Spartan Peptides LLC
All dosage protocols

GHK-Cu dosage chart

100 mg Vial

GHK-Cu (Copper Tripeptide-1) is a bioactive peptide known for its potential to support skin health, tissue repair, collagen production, and overall cellular regeneration. Spartan Peptides protocol: mix 100mg GHK-Cu with 3mL BAC water.

Mixing guide
Mix 100MG GHK-Cu with 3mL BAC water.
Concentration
33.3 mg/mL (~0.33mg per unit)
Recommended dosage
1mg – 5mg (recommended research range)
Injection frequency
Daily, or 5 days on / 2 days off
Syringe
Use an insulin syringe (U-100) for accurate dosing. 1 unit = 0.01 mL.
Storage
Store reconstituted vial refrigerated at 2–8 °C, protected from light. Do not freeze.

GHK-Cu dosing guide (100mg / 3mL BAC water)

Units (insulin syringe)MG (approximate)Recommended research range
3 units1mg1mg – 5mg
6 units2mg
9 units3mg
12 units4mg
15 units5mg

Reconstitution steps

  1. 1Draw 3mL of bacteriostatic water into a sterile syringe.
  2. 2Inject the water slowly down the inside wall of the 100MG GHK-Cu vial.
  3. 3Swirl gently until fully dissolved — do not shake.
  4. 4Use a U-100 insulin syringe for accurate dosing.
  5. 5Store reconstituted solution refrigerated at 2–8 °C, protected from light.

Spartan Peptides official protocol

Suggested pull: daily, or 5 days on / 2 days off.

  • Mixing guide: mix 100MG GHK-Cu with 3mL BAC water.
  • Concentration after mixing: 33.3 mg/mL (~0.33mg per unit).
  • Recommended dosage: 1mg – 5mg (recommended research range).
  • Injection frequency: Daily, or 5 days on / 2 days off.
  • Use an insulin syringe (U-100) for accurate dosing.

Tips to reduce stinging

Dilution example: pull 9 units (3mg) → dilute with 30–40 units BAC water → let sit 30 min at room temp before injecting.

  • After drawing your dose, dilute it with an additional 30–40 units of BAC water in the syringe.
  • Let the syringe sit at room temperature for about 30 minutes before injecting.
  • Inject slowly.
  • Glutes are preferred for less irritation.
  • Make sure alcohol fully dries before injecting.
  • Gentle massage after injection may help reduce sensitivity.

Reported research focus

Research purposes only. Premium quality. Built on legacy. Backed by science.

  • Supports skin health & tissue repair*
  • Supports collagen production*
  • Supports cellular regeneration*
  • Supports cognitive function*

Important Notes

Practical considerations for consistent research preparation and recordkeeping.

Published human GHK-Cu research has primarily evaluated topical and wound-care formulations, while local administration in an animal wound model provides additional preclinical context. The subcutaneous schedules above are research-use practice conventions, so findings should be interpreted according to route and formulation. Current clinical-trial and FDA materials provide additional topical, compounding, and regulatory context.

  • Use new sterile equipment for each preparation or administration and use a suitable sharps container.
  • Follow the written protocol for site selection and rotation.
  • Use aseptic technique and allow skin antiseptic to dry before administration.
  • Document the amount, date, preparation identifier, and site used.

How This Works

GHK-Cu is a naturally occurring copper-binding tripeptide studied in extracellular-matrix signaling, collagen-related pathways, antioxidant defense, and tissue remodeling. Human studies provide topical and wound-care context, while an animal wound model provides local-administration context.

Potential Benefits & Observed Effects

Findings reported in the GHK-Cu research literature.

  • Supports research into collagen-related signaling, extracellular-matrix remodeling, and tissue repair.
  • Human topical and wound-care studies have examined skin and tissue outcomes in formulation-specific settings.
  • Human-skin penetration research provides additional topical-delivery context.
  • Observed findings depend on route, formulation, concentration, and study design; the supporting context is summarized in Important Notes above.

Lifestyle Factors

Complementary approaches to support tissue health and regeneration.

  • Maintain adequate protein intake to support collagen synthesis and tissue repair processes.
  • Ensure sufficient hydration and micronutrient status (particularly copper, zinc, vitamin C) for optimal connective tissue metabolism.
  • Prioritize sleep quality and stress management to support regenerative biological processes.
  • Consider combining with appropriate skincare or wound-care protocols as relevant to individual goals.

Injection Technique

General subcutaneous technique references are included for preparation and measurement context.

  • Follow aseptic technique and use new sterile equipment for each administration.
  • Use the protocol-specified site, angle, and device; the CDC job aid illustrates general subcutaneous technique.
  • Rotate sites systematically and document each administration.
  • Dispose of used sharps immediately in an appropriate container.

important-note

This content is intended for therapeutic educational purposes only and does not constitute medical advice, diagnosis, or treatment.

GHK-Cu dosage FAQs

How do you reconstitute a 100 mg Vial vial of GHK-Cu?

Add bacteriostatic water slowly down the inside wall of the 100 mg Vial GHK-Cu vial, swirl gently until clear, and use the reconstitution calculator to convert your chosen water volume into syringe units.

What is a typical GHK-Cu dose?

Recommended dosage: 1mg – 5mg (recommended research range). Figures are research reference values only.

How often is GHK-Cu administered in research protocols?

Injection frequency: Daily, or 5 days on / 2 days off.

What concentration does reconstituted GHK-Cu end up at?

Concentration: 33.3 mg/mL (~0.33mg per unit). On any insulin syringe 1 unit = 0.01 mL, so unit counts convert directly across brands.

What does the GHK-Cu ghk-cu dosing guide (100mg / 3ml bac water) look like?

3 units — 1mg — 1mg – 5mg; 6 units — 2mg; 9 units — 3mg; 12 units — 4mg. The full table above lists every step with matching syringe units.

What are the mixing steps for GHK-Cu?

Draw 3mL of bacteriostatic water into a sterile syringe. Inject the water slowly down the inside wall of the 100MG GHK-Cu vial. Swirl gently until fully dissolved — do not shake.

How should reconstituted GHK-Cu be stored?

Storage: Store reconstituted vial refrigerated at 2–8 °C, protected from light. Do not freeze..

How many insulin syringe units equal a GHK-Cu dose?

Units depend on the water volume you used. Divide your target dose by the vial concentration to get millilitres, then multiply by 100 — 1 unit on a U-100 insulin syringe is 0.01 mL. The Spartan Peptides reconstitution calculator does this conversion for you.

Is GHK-Cu approved for human use?

No. GHK-Cu is sold and referenced strictly as a research chemical. Nothing on this page is medical advice and the material is not for human consumption.

Educational and research information only. Not medical advice, not for human consumption. Values are reference figures — always verify your own math with the reconstitution calculator.

On any insulin syringe, 1 unit = 0.01 mL, so unit counts above hold across brands.