Epitalon dosage chart
Epithalon) (10 mg Vial
Epitalon is dosed at 5 mg daily via subcutaneous injection in educational protocols. A Epithalon reconstituted with bacteriostatic water yields about 5 mg/mL. This information is for research and educational use only.
- Reconstitute
- Add 2.0 mL bacteriostatic water → 5 mg/mL concentration.
- Typical daily dose
- 5,000 mcg (5 mg) once daily for 20 consecutive days.
- Easy measuring
- At 5 mg/mL, 1 unit = 0.01 mL = 50 mcg on a U-100 insulin syringe.
- Storage
- Lyophilized: freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F); avoid freeze–thaw cycles.
- Injection frequency
- Once daily for 20 consecutive days — no rest days inside the cycle.
- Dosing schedule
- 20 consecutive days on, then off cycle. Cycles are typically repeated 2–3 times per year.
Dosing schedule
| Phase | Daily Dose (mcg) | Units (per injection) (mL) |
|---|---|---|
| Days 1–20 (Cycle On) | 5,000 mcg (5 mg) | 100 units (1.00 mL) |
| Weeks 4–26 (Cycle Off) | 0 mcg | — |
Reconstitution steps
- 1Draw 2.0 mL bacteriostatic water with a sterile syringe.
- 2Inject slowly down the vial wall; avoid foaming.
- 3Gently swirl/roll until dissolved (do not shake).
- 4Label and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.
Dosing Cycle & Timing
Epitalon uses a fixed 20-day continuous course rather than a weekly on/off cycle.
- Frequency: once daily for 20 consecutive days.
- Run all 20 days back to back — Epitalon is not cycled 5 days on / 2 days off.
- After day 20, stop and take an off-cycle break before repeating.
- Typical research pattern: a 20-day cycle repeated 2–3 times per year.
- Dose at roughly the same time each day, commonly in the evening.
Important Notes
Practical considerations for consistency and safety.
- Use new sterile insulin syringes; dispose in a sharps container.
- Rotate injection sites (abdomen, thighs, upper arms) to reduce local irritation.
- Inject slowly; wait a few seconds before withdrawing the needle.
- Document daily dose and site rotation to maintain consistency.
- Reconstitute one vial at a time (every 2 days) to keep solutions fresh.
How This Works
Epitalon’s primary mechanism involves telomerase activation, promoting telomere elongation in cells. In vitro studies demonstrated that Epitalon induced telomerase and lengthened telomeres in human cell cultures, allowing cells to continue dividing beyond the usual Hayflick limit. Epitalon also influences the neuroendocrine system by stimulating nocturnal melatonin release from the pineal gland, thereby normalizing circadian hormone rhythms in aged subjects. Additional reported effects include antioxidant activity, genomic regulatory effects, and immune modulation.
Potential Benefits & Side Effects
Observations from preclinical and clinical literature.
- Supports telomere maintenance and cellular longevity through telomerase activation.
- Restores healthy melatonin production and circadian rhythm regulation.
- Extended lifespan and reduced tumor incidence observed in animal models.
- Human trials showed improved cardiovascular outcomes and reduced mortality over 12-year follow-up.
- Generally well tolerated; no serious adverse events reported in long-term studies.
- Occasional mild injection-site reactions or transient sleep pattern changes may occur.
Lifestyle Factors
Complementary strategies for best outcomes.
- Prioritize sleep hygiene and maintain consistent sleep-wake schedules to complement melatonin effects.
- Pair with a balanced, antioxidant-rich diet to support cellular health.
- Engage in regular physical activity to reinforce metabolic and cardiovascular adaptations.
- Manage stress through mindfulness or relaxation techniques to support circadian balance.
Injection Technique
General subcutaneous guidance from clinical best-practice resources.
- Clean the vial stopper and skin with alcohol; allow to dry.
- Pinch a skinfold; insert the needle at 45–90° into subcutaneous tissue.
- Do not aspirate for subcutaneous injections; inject slowly and steadily.
- Rotate sites systematically (abdomen, thighs, upper arms) to avoid lipohypertrophy.
Important Note
This content is for educational purposes only and is not medical advice.
Supplies needed
Plan covers the full 20-day protocol at the lowest listed starting dose: 5 mg per dose, once daily (20 administrations in total), using 10 mg vials. After mixing with 2 mL, the concentration is 5 mg/mL and each dose requires 1 mL total.
Epitalon vials (Epithalon) (10 mg each)
Vials are rounded up only after calculating the whole protocol: ceil(100 ÷ 10) = 10. Usable peptide remaining in an opened vial carries forward to the next dose.
- Full protocol (20 days)
- 10 vials (100 mg needed ÷ 10 mg)
Insulin syringes (U-100, 1 mL)
One fresh syringe per administration.
- Full protocol (20 days)
- 20 syringes
Bacteriostatic water (10 mL bottles)
Use 2 mL per vial for reconstitution (10 × 2 mL = 20 mL).
- Full protocol (20 days)
- 2 × 10 mL bottles
Alcohol swabs
One for the vial stopper per draw + one for each injection site (once daily).
- Full protocol (20 days)
- 40 swabs
| Supply | Full protocol (20 days) |
|---|---|
| Epitalon vials (Epithalon) (10 mg each)Vials are rounded up only after calculating the whole protocol: ceil(100 ÷ 10) = 10. Usable peptide remaining in an opened vial carries forward to the next dose. | 10 vials (100 mg needed ÷ 10 mg) |
| Insulin syringes (U-100, 1 mL)One fresh syringe per administration. | 20 syringes |
| Bacteriostatic water (10 mL bottles)Use 2 mL per vial for reconstitution (10 × 2 mL = 20 mL). | 2 × 10 mL bottles |
| Alcohol swabsOne for the vial stopper per draw + one for each injection site (once daily). | 40 swabs |
Epitalon dosage FAQs
How much bacteriostatic water do you use to reconstitute Epitalon?
Add 2.0 mL bacteriostatic water → 5 mg/mL concentration. for a Epithalon) (10 mg Vial vial. Add the water slowly against the inside wall of the vial and swirl — never shake — until the powder fully dissolves.
What is a typical Epitalon dose?
Typical daily dose: 5,000 mcg (5 mg) once daily for 20 consecutive days.. Figures are research reference values only.
How often is Epitalon administered in research protocols?
Injection frequency: Once daily for 20 consecutive days — no rest days inside the cycle.
What does the Epitalon dosing schedule look like?
Days 1–20 (Cycle On) — 5,000 mcg (5 mg) — 100 units (1.00 mL); Weeks 4–26 (Cycle Off) — 0 mcg — —. The full table above lists every step with matching syringe units.
What are the mixing steps for Epitalon?
Draw 2.0 mL bacteriostatic water with a sterile syringe. Inject slowly down the vial wall; avoid foaming. Gently swirl/roll until dissolved (do not shake).
How should reconstituted Epitalon be stored?
Storage: Lyophilized: freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F); avoid freeze–thaw cycles..
How many insulin syringe units equal a Epitalon dose?
Units depend on the water volume you used. Divide your target dose by the vial concentration to get millilitres, then multiply by 100 — 1 unit on a U-100 insulin syringe is 0.01 mL. The Spartan Peptides reconstitution calculator does this conversion for you.
Is Epitalon approved for human use?
No. Epitalon is sold and referenced strictly as a research chemical. Nothing on this page is medical advice and the material is not for human consumption.
Educational and research information only. Not medical advice, not for human consumption. Values are reference figures — always verify your own math with the reconstitution calculator.
On any insulin syringe, 1 unit = 0.01 mL, so unit counts above hold across brands.