DSIP dosage chart
5 mg Vial
DSIP (Delta Sleep-Inducing Peptide) is dosed at 100 mcg–300 mcg daily via subcutaneous injection in educational protocols, usually before bedtime. A 5 mg vial reconstituted with bacteriostatic water yields about 1.67 mg/mL. This information is for research and educational use only.
- Reconstitute
- Add 3.0 mL bacteriostatic water → ~1.67 mg/mL concentration.
- Typical daily range
- 100–300 mcg once daily (gradual titration); advanced up to 500 mcg.
- Easy measuring
- At 1.67 mg/mL, 1 unit = 0.01 mL ≈ 16.7 mcg on a U-100 insulin syringe.
- Storage
- Lyophilized: freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F); avoid freeze–thaw cycles.
- Injection frequency
- Once daily — 5 days on, 2 days off (dose Mon–Fri, rest Sat–Sun).
- Dosing schedule
- 5 days on / 2 days off: dose Monday through Friday, then take Saturday and Sunday off. That is 5 dosing days per week.
Dosing schedule
| Week | Daily Dose (mcg) | Units (per injection) (mL) |
|---|---|---|
| Week 1 | 100 mcg | 6 units (0.06 mL) |
| Week 2 | 150 mcg | 9 units (0.09 mL) |
| Week 3 | 200 mcg | 12 units (0.12 mL) |
| Weeks 4–8 | 250–300 mcg | 15–18 units (0.15–0.18 mL) |
Advanced / Extended Approach
| Week | Daily Dose (mcg) | Units (per injection) (mL) |
|---|---|---|
| Week 5 | 350–400 mcg | 21–24 units (0.21–0.24 mL) |
| Weeks 6–8+ | 400–500 mcg | 24–30 units (0.24–0.30 mL) |
Reconstitution steps
- 1Draw 3.0 mL bacteriostatic water with a sterile syringe.
- 2Inject slowly down the vial wall; avoid foaming.
- 3Gently swirl/roll until dissolved (do not shake).
- 4Label and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.
Dosing Cycle & Timing
This protocol runs on a 5 days on / 2 days off cycle.
- Cycle: 5 days on, 2 days off — dose Monday through Friday and rest on the weekend.
- 5 dosing days per week; the 2 off days help limit receptor desensitisation and give injection sites time to recover.
- Keep the dose time consistent on dosing days.
- If a weekday dose is missed, take it the same day if possible — do not dose on an off day to catch up.
- Weekly totals in the supplies and shopping lists are calculated on 5 dosing days, not 7.
Important Notes
Practical considerations for consistency and safety.
- Use new sterile insulin syringes; dispose in a sharps container.
- Rotate injection sites (abdomen, thighs, upper arms) to reduce local irritation.
- Inject slowly; wait a few seconds before withdrawing the needle.
- Document daily dose and site rotation to maintain consistency.
- Some research suggests DSIP effects may persist for multiple nights after dosing; adjust frequency as needed.
How This Works
DSIP (Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu) was first characterized in 1977 as a sleep-modulating factor isolated from rabbit brain. Mechanistic studies suggest DSIP may influence sleep by modulating GABAergic transmission and interacting with opioid receptor systems. Research indicates it can increase delta-wave (slow-wave) sleep duration without significantly altering REM sleep architecture. Beyond sleep, DSIP has demonstrated stress-protective and adaptogenic properties in various models, potentially through modulation of the hypothalamic-pituitary-adrenal axis.
Potential Benefits & Side Effects
Observations from preclinical and clinical literature.
- May promote deeper, more restorative slow-wave sleep and improve subjective sleep quality.
- Research suggests potential stress-protective and anxiolytic-like effects.
- Some studies indicate DSIP does not induce pharmacological tolerance with continued use.
- Generally well tolerated in human studies; occasional mild injection-site reactions may occur with subcutaneous administration.
- Limited long-term safety data; periodic breaks are recommended as a precaution.
Lifestyle Factors
Complementary strategies for best outcomes.
- Maintain consistent sleep-wake schedules to reinforce circadian rhythms.
- Limit blue light exposure and stimulants (caffeine, nicotine) in the hours before bed.
- Create a cool, dark, quiet sleep environment.
- Incorporate regular physical activity, ideally earlier in the day.
- Manage stress through relaxation techniques (meditation, deep breathing).
Injection Technique
General subcutaneous guidance from clinical best-practice resources.
- Clean the vial stopper and skin with alcohol; allow to dry.
- Pinch a skinfold; insert the needle at 45–90° into subcutaneous tissue.
- Do not aspirate for subcutaneous injections; inject slowly and steadily.
- Rotate sites systematically (abdomen at least 2 inches from navel, thighs, upper arms) to avoid lipohypertrophy.
Important Note
This content is for educational purposes only and is not medical advice.
Supplies needed
Plan covers the full 8-week protocol at the lowest listed starting dose: 0.1 mg per dose, 5 days on / 2 days off (40 administrations in total), using 5 mg vials. After mixing with 3 mL, the concentration is 1.67 mg/mL and each dose requires 0.06 mL total.
DSIP vials (5 mg each)
Vials are rounded up only after calculating the whole protocol: ceil(4 ÷ 5) = 1. Usable peptide remaining in an opened vial carries forward to the next dose.
- Full protocol (8 weeks)
- 1 vial (4 mg needed ÷ 5 mg)
Insulin syringes (U-100, 1 mL)
One fresh syringe per administration.
- Full protocol (8 weeks)
- 40 syringes
Bacteriostatic water (10 mL bottles)
Use 3 mL per vial for reconstitution (1 × 3 mL = 3 mL).
- Full protocol (8 weeks)
- 1 × 10 mL bottle
Alcohol swabs
One for the vial stopper per draw + one for each injection site (5 days on / 2 days off). Recommend 1 × 100-count box.
- Full protocol (8 weeks)
- 80 swabs
| Supply | Full protocol (8 weeks) |
|---|---|
| DSIP vials (5 mg each)Vials are rounded up only after calculating the whole protocol: ceil(4 ÷ 5) = 1. Usable peptide remaining in an opened vial carries forward to the next dose. | 1 vial (4 mg needed ÷ 5 mg) |
| Insulin syringes (U-100, 1 mL)One fresh syringe per administration. | 40 syringes |
| Bacteriostatic water (10 mL bottles)Use 3 mL per vial for reconstitution (1 × 3 mL = 3 mL). | 1 × 10 mL bottle |
| Alcohol swabsOne for the vial stopper per draw + one for each injection site (5 days on / 2 days off). Recommend 1 × 100-count box. | 80 swabs |
DSIP dosage FAQs
How much bacteriostatic water do you use to reconstitute DSIP?
Add 3.0 mL bacteriostatic water → ~1.67 mg/mL concentration. for a 5 mg Vial vial. Add the water slowly against the inside wall of the vial and swirl — never shake — until the powder fully dissolves.
How often is DSIP administered in research protocols?
Injection frequency: Once daily — 5 days on, 2 days off (dose Mon–Fri, rest Sat–Sun).
What does the DSIP dosing schedule look like?
Week 1 — 100 mcg — 6 units (0.06 mL); Week 2 — 150 mcg — 9 units (0.09 mL); Week 3 — 200 mcg — 12 units (0.12 mL); Weeks 4–8 — 250–300 mcg — 15–18 units (0.15–0.18 mL). The full table above lists every step with matching syringe units.
What are the mixing steps for DSIP?
Draw 3.0 mL bacteriostatic water with a sterile syringe. Inject slowly down the vial wall; avoid foaming. Gently swirl/roll until dissolved (do not shake).
How should reconstituted DSIP be stored?
Storage: Lyophilized: freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F); avoid freeze–thaw cycles..
How many insulin syringe units equal a DSIP dose?
Units depend on the water volume you used. Divide your target dose by the vial concentration to get millilitres, then multiply by 100 — 1 unit on a U-100 insulin syringe is 0.01 mL. The Spartan Peptides reconstitution calculator does this conversion for you.
Is DSIP approved for human use?
No. DSIP is sold and referenced strictly as a research chemical. Nothing on this page is medical advice and the material is not for human consumption.
Educational and research information only. Not medical advice, not for human consumption. Values are reference figures — always verify your own math with the reconstitution calculator.
On any insulin syringe, 1 unit = 0.01 mL, so unit counts above hold across brands.