CJC-1295 NO DAC dosage chart

5 mg Vial

CJC-1295 NO DAC is dosed at 100 mcg–300 mcg daily via subcutaneous injection in educational protocols. A 5 mg vial reconstituted with bacteriostatic water yields about 1.67 mg/mL. This information is for research and educational use only.

Reconstitute: Add 3.0 mL bacteriostatic water → ~1.67 mg/mL concentration.
Typical daily range: 100–300 mcg once daily (gradual titration).
Easy measuring: At 1.67 mg/mL, 1 unit = 0.01 mL ≈ 16.7 mcg on a U‑100 insulin syringe.
Storage: Lyophilized: refrigerate at 2–8 °C (35.6–46.4 °F); after reconstitution, refrigerate and use within 1–2 weeks; for longer storage, freeze at ≤−20 °C (≤−4 °F).
Injection frequency: Once daily — 5 days on, 2 days off (dose Mon–Fri, rest Sat–Sun).
Dosing schedule: 5 days on / 2 days off: dose Monday through Friday, then take Saturday and Sunday off. That is 5 dosing days per week.

Dosing schedule

Week	Daily Dose (mcg)	Units (per injection) (mL)
Weeks 1–2	100 mcg	6 units (0.06 mL)
Weeks 3–4	150 mcg	9 units (0.09 mL)
Weeks 5–6	200 mcg	12 units (0.12 mL)
Weeks 7–12	250–300 mcg	15–18 units (0.15–0.18 mL)

Reconstitution steps

1Draw 3.0 mL bacteriostatic water with a sterile syringe.
2Inject slowly down the vial wall; avoid foaming.
3Gently swirl/roll until dissolved (do not shake).
4Label and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.

Dosing Cycle & Timing

This protocol runs on a 5 days on / 2 days off cycle.

Cycle: 5 days on, 2 days off — dose Monday through Friday and rest on the weekend.
5 dosing days per week; the 2 off days help limit receptor desensitisation and give injection sites time to recover.
Keep the dose time consistent on dosing days.
If a weekday dose is missed, take it the same day if possible — do not dose on an off day to catch up.
Weekly totals in the supplies and shopping lists are calculated on 5 dosing days, not 7.

Important Notes

Practical considerations for consistency and safety.

Use new sterile insulin syringes; dispose in a sharps container.
Rotate injection sites (abdomen, thighs, upper arms) to reduce local irritation.
Inject slowly; wait a few seconds before withdrawing the needle.
Document daily dose and site rotation to maintain consistency.
Administer on an empty stomach (fasted for ≥2 hours) to optimize GH response.

How This Works

CJC-1295 NO DAC (Modified GRF 1–29) is a truncated GHRH analog with four amino acid substitutions (D‑Ala, Gln, Ala, Leu) that enhance stability against enzymatic degradation. It binds to GHRH receptors on pituitary somatotrophs, stimulating the synthesis and pulsatile release of endogenous growth hormone. Unlike exogenous GH administration, GHRH analogs preserve the physiologic feedback loop, resulting in more natural GH pulsatility without the risks associated with supraphysiological GH levels. The short half‑life (~30 minutes) produces discrete GH pulses that mimic the body’s natural secretion pattern.

Potential Benefits & Side Effects

Observations from preclinical and clinical literature on GHRH analogs.

Stimulates pulsatile GH release and elevates IGF‑1 levels with chronic administration.
May support improvements in body composition, including increased lean mass and reduced fat mass.
May enhance recovery, sleep quality, and overall energy levels.
Generally well tolerated; occasional mild side effects may include injection‑site reactions (redness, swelling), transient flushing, or headache.

Lifestyle Factors

Complementary strategies for best outcomes.

Pair with a balanced, protein‑forward diet tailored to energy needs.
Combine resistance training and aerobic activity to reinforce metabolic adaptations and GH response.
Prioritize sleep and stress management to support natural GH pulsatility and recovery.
Administer peptide in a fasted state to maximize GH release.

Injection Technique

General subcutaneous guidance from clinical best‑practice resources.

Clean the vial stopper and skin with alcohol; allow to dry.
Pinch a skinfold; insert the needle at 45–90° into subcutaneous tissue.
Do not aspirate for subcutaneous injections; inject slowly and steadily.
Rotate sites systematically (abdomen, thighs, upper arms) to avoid lipohypertrophy.

Important Note

This content is intended for therapeutic educational purposes only and does not constitute medical advice, diagnosis, or treatment.

Supplies needed

Plan covers the full 12-week protocol at the lowest listed starting dose: 0.1 mg per dose, 5 days on / 2 days off (60 administrations in total), using 5 mg vials. After mixing with 3 mL, the concentration is 1.67 mg/mL and each dose requires 0.06 mL total.

CJC-1295 NO DAC vials (5 mg each)

Vials are rounded up only after calculating the whole protocol: ceil(6 ÷ 5) = 2. Usable peptide remaining in an opened vial carries forward to the next dose.

Full protocol (12 weeks): 2 vials (6 mg needed ÷ 5 mg)

Insulin syringes (U-100, 1 mL)

One fresh syringe per administration.

Full protocol (12 weeks): 60 syringes

Bacteriostatic water (10 mL bottles)

Use 3 mL per vial for reconstitution (2 × 3 mL = 6 mL).

Full protocol (12 weeks): 1 × 10 mL bottle

Alcohol swabs

One for the vial stopper per draw + one for each injection site (5 days on / 2 days off). Recommend 2 × 100-count boxes.

Full protocol (12 weeks): 120 swabs

Supply	Full protocol (12 weeks)
CJC-1295 NO DAC vials (5 mg each)Vials are rounded up only after calculating the whole protocol: ceil(6 ÷ 5) = 2. Usable peptide remaining in an opened vial carries forward to the next dose.	2 vials (6 mg needed ÷ 5 mg)
Insulin syringes (U-100, 1 mL)One fresh syringe per administration.	60 syringes
Bacteriostatic water (10 mL bottles)Use 3 mL per vial for reconstitution (2 × 3 mL = 6 mL).	1 × 10 mL bottle
Alcohol swabsOne for the vial stopper per draw + one for each injection site (5 days on / 2 days off). Recommend 2 × 100-count boxes.	120 swabs

CJC-1295 NO DAC dosage FAQs

How much bacteriostatic water do you use to reconstitute CJC-1295 NO DAC?

Add 3.0 mL bacteriostatic water → ~1.67 mg/mL concentration. for a 5 mg Vial vial. Add the water slowly against the inside wall of the vial and swirl — never shake — until the powder fully dissolves.

How often is CJC-1295 NO DAC administered in research protocols?

Injection frequency: Once daily — 5 days on, 2 days off (dose Mon–Fri, rest Sat–Sun).

What does the CJC-1295 NO DAC dosing schedule look like?

Weeks 1–2 — 100 mcg — 6 units (0.06 mL); Weeks 3–4 — 150 mcg — 9 units (0.09 mL); Weeks 5–6 — 200 mcg — 12 units (0.12 mL); Weeks 7–12 — 250–300 mcg — 15–18 units (0.15–0.18 mL). The full table above lists every step with matching syringe units.

What are the mixing steps for CJC-1295 NO DAC?

Draw 3.0 mL bacteriostatic water with a sterile syringe. Inject slowly down the vial wall; avoid foaming. Gently swirl/roll until dissolved (do not shake).

How should reconstituted CJC-1295 NO DAC be stored?

Storage: Lyophilized: refrigerate at 2–8 °C (35.6–46.4 °F); after reconstitution, refrigerate and use within 1–2 weeks; for longer storage, freeze at ≤−20 °C (≤−4 °F)..

How many insulin syringe units equal a CJC-1295 NO DAC dose?

Units depend on the water volume you used. Divide your target dose by the vial concentration to get millilitres, then multiply by 100 — 1 unit on a U-100 insulin syringe is 0.01 mL. The Spartan Peptides reconstitution calculator does this conversion for you.

Is CJC-1295 NO DAC approved for human use?

No. CJC-1295 NO DAC is sold and referenced strictly as a research chemical. Nothing on this page is medical advice and the material is not for human consumption.

Educational and research information only. Not medical advice, not for human consumption. Values are reference figures — always verify your own math with the reconstitution calculator.

On any insulin syringe, 1 unit = 0.01 mL, so unit counts above hold across brands.