BPC-157 + TB-500 dosage chart
10 mg Blend
The BPC-157 + TB-500 peptide blend is dosed at 600 mcg–800 mcg daily via subcutaneous injection in educational protocols. A 10 mg blend reconstituted with bacteriostatic water yields about 3.33 mg/mL. This information is for research and educational use only.
- Reconstitute
- Add 3.0 mL bacteriostatic water → 3.33 mg/mL total concentration (1.67 mg/mL of each peptide).
- Typical daily range
- 600–1000 mcg total blend once daily (provides 300–500 mcg of each peptide).
- Easy measuring
- At 3.33 mg/mL, 1 unit = 0.01 mL ≈ 33.3 mcg total blend on a U-100 insulin syringe.
- Storage
- Lyophilized: freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F); avoid freeze–thaw cycles.
- Injection frequency
- Once daily — 5 days on, 2 days off (dose Mon–Fri, rest Sat–Sun).
- Dosing schedule
- 5 days on / 2 days off: dose Monday through Friday, then take Saturday and Sunday off. That is 5 dosing days per week.
Standard / Gradual Approach (3 mL = ~3.33 mg/mL)
| PHASE / WEEK | DAILY DOSE (TOTAL BLEND) | EACH PEPTIDE (MCG) | UNITS (ML) |
|---|---|---|---|
| Weeks 1–2 (Initial) | 600 mcg | 300 mcg BPC + 300 mcg TB-500 | 18 units (0.18 mL) |
| Weeks 3–4 (Loading) | 800 mcg | 400 mcg BPC + 400 mcg TB-500 | 24 units (0.24 mL) |
| Weeks 5–8 (Maintenance) | 600 mcg | 300 mcg BPC + 300 mcg TB-500 | 18 units (0.18 mL) |
Advanced / Aggressive Approach (Acute Injury Support)
| PHASE / WEEK | DAILY DOSE (TOTAL BLEND) | EACH PEPTIDE (MCG) | UNITS (ML) |
|---|---|---|---|
| Weeks 1–2 (Aggressive Load) | 1000 mcg | 500 mcg BPC + 500 mcg TB-500 | 30 units (0.30 mL) |
| Weeks 3–4 (High Load) | 800 mcg | 400 mcg BPC + 400 mcg TB-500 | 24 units (0.24 mL) |
| Weeks 5–8 (Maintenance) | 600 mcg | 300 mcg BPC + 300 mcg TB-500 | 18 units (0.18 mL) |
Reconstitution steps
- 1Draw 3.0 mL bacteriostatic water with a sterile syringe.
- 2Inject slowly down the vial wall; avoid foaming or vigorous shaking.
- 3Gently swirl/roll until fully dissolved (do not shake).
- 4Label with date and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.
Dosing Cycle & Timing
This protocol runs on a 5 days on / 2 days off cycle.
- Cycle: 5 days on, 2 days off — dose Monday through Friday and rest on the weekend.
- 5 dosing days per week; the 2 off days help limit receptor desensitisation and give injection sites time to recover.
- Keep the dose time consistent on dosing days.
- If a weekday dose is missed, take it the same day if possible — do not dose on an off day to catch up.
- Weekly totals in the supplies and shopping lists are calculated on 5 dosing days, not 7.
Protocol Overview
Concise summary of the once-daily regimen.
- Goal: Support tissue repair, wound healing, and recovery from musculoskeletal injuries.
- Schedule: Daily subcutaneous injections for 8–12 weeks (extend to 16 weeks if needed).
- Dose Range: 600–1000 mcg total blend daily (300–500 mcg of each peptide).
- Reconstitution: 3.0 mL per 10 mg vial (~3.33 mg/mL) for accurate unit measurements.
- Storage: Lyophilized frozen; reconstituted refrigerated; avoid repeated freeze–thaw.
Dosing Protocol
Suggested daily phased approach.
- Start: 600 mcg total daily (300 mcg each peptide) for Weeks 1–2.
- Loading: Increase to 800 mcg daily for Weeks 3–4.
- Maintenance: Return to 600 mcg daily for Weeks 5–8+.
- Frequency: Once per day (subcutaneous).
- Cycle Length: 8–12 weeks; optional extension to 16 weeks.
Storage Instructions
Proper storage preserves peptide quality and stability.
- Lyophilized: Store at −20 °C (−4 °F) in dry, dark conditions; minimize moisture exposure.
- Reconstituted: Refrigerate at 2–8 °C (35.6–46.4 °F); use within 4–6 weeks; avoid freeze–thaw.
- Allow vials to reach room temperature before opening to reduce condensation uptake.
Important Notes
Practical considerations for consistency and safety.
- Both peptides are not approved for routine human use (sold for research purposes) and are banned in competitive sports.
- Use new sterile insulin syringes for each injection; dispose in a sharps container.
- Rotate injection sites (abdomen, thighs, upper arms) to reduce local irritation.
- Inject slowly; wait a few seconds before withdrawing the needle.
- Document daily dose and site rotation to maintain consistency.
How This Works
BPC-157 is a stable pentadecapeptide that promotes angiogenesis, modulates nitric oxide pathways, and demonstrates broad cytoprotective effects across gastrointestinal, musculoskeletal, and neurological tissues in preclinical models. It has shown activity at very low doses (nanogram to microgram per kilogram) without demonstrable toxicity in animal studies.
TB-500 (Thymosin Beta-4) is an actin-sequestering peptide that promotes cell migration, wound healing, and anti-inflammatory responses. It is well-tolerated in animal and early clinical studies, even at multi-milligram doses.
Combining these peptides may provide complementary mechanisms for tissue repair: BPC-157 for its trophic and anti-inflammatory effects, and TB-500 for enhanced cell migration and angiogenesis.
Potential Benefits & Side Effects
Observations from preclinical and limited clinical literature.
- May support accelerated healing of tendons, ligaments, muscles, and soft tissue injuries.
- Preclinical evidence suggests gastroprotective and anti-inflammatory properties for BPC-157.
- TB-500 promotes wound healing and may reduce scarring through enhanced cell migration.
- Both peptides are generally well tolerated; occasional mild injection-site reactions (redness, itching) may occur with subcutaneous administration.
- No significant toxicity has been documented up to high doses in animal studies.
Lifestyle Factors
Complementary strategies for optimal recovery outcomes.
- Prioritize adequate protein intake (1.6–2.2 g/kg body weight) to support tissue repair.
- Follow appropriate rehabilitation protocols for any injuries being addressed.
- Ensure adequate sleep (7–9 hours) to optimize recovery and tissue regeneration.
- Manage inflammation through balanced nutrition and stress reduction techniques.
Injection Technique
General subcutaneous guidance from clinical best-practice resources.
- Clean the vial stopper and skin with alcohol; allow to dry completely.
- Pinch a skinfold; insert the needle at 45–90° into subcutaneous tissue.
- Do not aspirate for subcutaneous injections; inject slowly and steadily.
- Rotate sites systematically (abdomen at least 2 inches from navel, thighs, upper arms, flank) to avoid lipohypertrophy.
- Apply gentle pressure post-injection; do not rub the site.
Important Note
This content is for educational purposes only and is not medical advice.
Supplies needed
Plan covers the full 8-week protocol at the lowest listed starting dose: 0.6 mg per dose, 5 days on / 2 days off (40 administrations in total), using 10 mg vials. After mixing with 3 mL, the concentration is 3.33 mg/mL and each dose requires 0.18 mL total.
BPC-157 + TB-500 vials (10 mg Blend each)
Vials are rounded up only after calculating the whole protocol: ceil(24 ÷ 10) = 3. Usable peptide remaining in an opened vial carries forward to the next dose.
- Full protocol (8 weeks)
- 3 vials (24 mg needed ÷ 10 mg)
Insulin syringes (U-100, 1 mL)
One syringe per draw — some doses need more than one draw (dose exceeds a single 1 mL syringe or finishes a vial).
- Full protocol (8 weeks)
- 42 syringes
Bacteriostatic water (10 mL bottles)
Use 3 mL per vial for reconstitution (3 × 3 mL = 9 mL).
- Full protocol (8 weeks)
- 1 × 10 mL bottle
Alcohol swabs
One for the vial stopper per draw + one for each injection site (5 days on / 2 days off). Recommend 1 × 100-count box.
- Full protocol (8 weeks)
- 82 swabs
| Supply | Full protocol (8 weeks) |
|---|---|
| BPC-157 + TB-500 vials (10 mg Blend each)Vials are rounded up only after calculating the whole protocol: ceil(24 ÷ 10) = 3. Usable peptide remaining in an opened vial carries forward to the next dose. | 3 vials (24 mg needed ÷ 10 mg) |
| Insulin syringes (U-100, 1 mL)One syringe per draw — some doses need more than one draw (dose exceeds a single 1 mL syringe or finishes a vial). | 42 syringes |
| Bacteriostatic water (10 mL bottles)Use 3 mL per vial for reconstitution (3 × 3 mL = 9 mL). | 1 × 10 mL bottle |
| Alcohol swabsOne for the vial stopper per draw + one for each injection site (5 days on / 2 days off). Recommend 1 × 100-count box. | 82 swabs |
BPC-157 + TB-500 dosage FAQs
How much bacteriostatic water do you use to reconstitute BPC-157 + TB-500?
Add 3.0 mL bacteriostatic water → 3.33 mg/mL total concentration (1.67 mg/mL of each peptide). for a 10 mg Blend vial. Add the water slowly against the inside wall of the vial and swirl — never shake — until the powder fully dissolves.
How often is BPC-157 + TB-500 administered in research protocols?
Injection frequency: Once daily — 5 days on, 2 days off (dose Mon–Fri, rest Sat–Sun).
What does the BPC-157 + TB-500 standard / gradual approach (3 ml = ~3.33 mg/ml) look like?
Weeks 1–2 (Initial) — 600 mcg — 300 mcg BPC + 300 mcg TB-500 — 18 units (0.18 mL); Weeks 3–4 (Loading) — 800 mcg — 400 mcg BPC + 400 mcg TB-500 — 24 units (0.24 mL); Weeks 5–8 (Maintenance) — 600 mcg — 300 mcg BPC + 300 mcg TB-500 — 18 units (0.18 mL). The full table above lists every step with matching syringe units.
What are the mixing steps for BPC-157 + TB-500?
Draw 3.0 mL bacteriostatic water with a sterile syringe. Inject slowly down the vial wall; avoid foaming or vigorous shaking. Gently swirl/roll until fully dissolved (do not shake).
How should reconstituted BPC-157 + TB-500 be stored?
Storage: Lyophilized: freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F); avoid freeze–thaw cycles..
How many insulin syringe units equal a BPC-157 + TB-500 dose?
Units depend on the water volume you used. Divide your target dose by the vial concentration to get millilitres, then multiply by 100 — 1 unit on a U-100 insulin syringe is 0.01 mL. The Spartan Peptides reconstitution calculator does this conversion for you.
Is BPC-157 + TB-500 approved for human use?
No. BPC-157 + TB-500 is sold and referenced strictly as a research chemical. Nothing on this page is medical advice and the material is not for human consumption.
Educational and research information only. Not medical advice, not for human consumption. Values are reference figures — always verify your own math with the reconstitution calculator.
On any insulin syringe, 1 unit = 0.01 mL, so unit counts above hold across brands.