Spartan Peptides LLC
All dosage protocols

AOD 9604 dosage chart

5 mg Vial

AOD 9604 is a modified fragment of human growth hormone (HGH) designed to support fat metabolism and may aid in weight management. Spartan Peptides protocol: mix 5mg AOD 9604 with 2mL BAC water.

Mixing guide
Mix 5MG AOD 9604 with 2mL BAC water.
Concentration
2.5 mg/mL
Recommended dosage
250mcg – 500mcg per day
Injection frequency
1x daily (recommended fasted)
Syringe
Use an insulin syringe (U-100) for accurate dosing. 1 unit = 0.01 mL.
Storage
Store reconstituted vial refrigerated at 2–8 °C, protected from light. Do not freeze.

AOD 9604 dosing guide (5mg / 2mL BAC water)

Units (insulin syringe)AmountRecommended range
10 units250mcg250mcg – 500mcg
12 units300mcg
14 units350mcg
16 units400mcg
18 units450mcg
20 units500mcg

Reconstitution steps

  1. 1Draw 2mL of bacteriostatic water into a sterile syringe.
  2. 2Inject the water slowly down the inside wall of the 5MG AOD 9604 vial.
  3. 3Swirl gently until fully dissolved — do not shake.
  4. 4Use a U-100 insulin syringe for accurate dosing.
  5. 5Store reconstituted solution refrigerated at 2–8 °C, protected from light.

Spartan Peptides official protocol

All doses are 1x daily.

  • Mixing guide: mix 5MG AOD 9604 with 2mL BAC water.
  • Concentration after mixing: 2.5 mg/mL.
  • Recommended dosage: 250mcg – 500mcg per day.
  • Injection frequency: 1x daily (recommended fasted).
  • Use an insulin syringe (U-100) for accurate dosing.

Reported research focus

Research purposes only. Premium quality. Built on legacy. Backed by science.

  • Supports fat metabolism*
  • Supports weight management*
  • Enhances recovery*
  • Supports overall wellness*

Important Notes

Practical considerations for consistency and safety.

  • Use new sterile insulin syringes (U‑100, 29–31 gauge); dispose in a sharps container.
  • Rotate injection sites (abdomen, thighs, upper arms) to reduce local irritation and prevent lipohypertrophy.
  • Inject slowly; wait a few seconds before withdrawing the needle.
  • Each 5 mg vial provides approximately 10 days of dosing at 500 mcg/day or 16–17 days at 300 mcg/day.

How This Works

AOD‑9604 is a modified C‑terminal fragment of human growth hormone that retains the lipolytic (fat‑reducing) domain without the growth‑promoting effects. It binds to adipose tissue and triggers breakdown of stored fat while blocking new fat storage (re‑esterification) in adipocytes. At the molecular level, chronic AOD‑9604 administration upregulates β3‑adrenergic receptors in fat tissue, reversing obesity‑related suppression of these fat‑burning receptors. Unlike full‑length hGH, AOD‑9604 does not meaningfully elevate IGF‑1 levels or worsen glucose tolerance, making its tolerability indistinguishable from placebo in human trials.

Potential Benefits & Side Effects

Observations from preclinical and clinical literature.

  • Supports reductions in fat mass and increases in fat oxidation over time; clinical trials showed modest but statistically significant weight loss (approximately 2.6 kg vs. 0.8 kg placebo over 12 weeks at 1 mg/day).
  • Preferential loss of abdominal fat observed, resembling the pattern seen with low‑dose hGH therapy.
  • Does not meaningfully raise IGF‑1 and shows a placebo‑like safety profile in human studies; no anti‑AOD9604 antibodies detected.
  • Emerging regenerative potential: preclinical studies suggest possible cartilage repair and bone health benefits.
  • Generally well tolerated; occasional mild injection‑site reactions (redness/itch) may occur with subcutaneous administration.

Lifestyle Factors

Complementary strategies for best outcomes.

  • Pair with a hypocaloric, protein‑forward diet tailored to energy needs; AOD‑9604 is best viewed as a supportive aid to fat loss rather than a stand‑alone solution.
  • Combine resistance training and aerobic activity to reinforce metabolic adaptations.
  • Prioritize sleep and stress management to support adherence and recovery.

Injection Technique

General subcutaneous guidance from clinical best‑practice resources.

  • Clean the vial stopper and skin with alcohol; allow to dry completely.
  • Pinch a skinfold; insert the needle at 90° (or 45° if very little subcutaneous fat) into subcutaneous tissue.
  • Do not aspirate for subcutaneous injections; inject slowly and steadily.
  • Rotate sites systematically (abdomen at least 2 inches from navel, thighs, upper arms) to avoid lipohypertrophy.

important-note

This content is intended for therapeutic educational purposes only and does not constitute medical advice, diagnosis, or treatment.

AOD 9604 dosage FAQs

How do you reconstitute a 5 mg Vial vial of AOD 9604?

Add bacteriostatic water slowly down the inside wall of the 5 mg Vial AOD 9604 vial, swirl gently until clear, and use the reconstitution calculator to convert your chosen water volume into syringe units.

What is a typical AOD 9604 dose?

Recommended dosage: 250mcg – 500mcg per day. Figures are research reference values only.

How often is AOD 9604 administered in research protocols?

Injection frequency: 1x daily (recommended fasted).

What concentration does reconstituted AOD 9604 end up at?

Concentration: 2.5 mg/mL. On any insulin syringe 1 unit = 0.01 mL, so unit counts convert directly across brands.

What does the AOD 9604 aod 9604 dosing guide (5mg / 2ml bac water) look like?

10 units — 250mcg — 250mcg – 500mcg; 12 units — 300mcg; 14 units — 350mcg; 16 units — 400mcg. The full table above lists every step with matching syringe units.

What are the mixing steps for AOD 9604?

Draw 2mL of bacteriostatic water into a sterile syringe. Inject the water slowly down the inside wall of the 5MG AOD 9604 vial. Swirl gently until fully dissolved — do not shake.

How should reconstituted AOD 9604 be stored?

Storage: Store reconstituted vial refrigerated at 2–8 °C, protected from light. Do not freeze..

How many insulin syringe units equal a AOD 9604 dose?

Units depend on the water volume you used. Divide your target dose by the vial concentration to get millilitres, then multiply by 100 — 1 unit on a U-100 insulin syringe is 0.01 mL. The Spartan Peptides reconstitution calculator does this conversion for you.

Is AOD 9604 approved for human use?

No. AOD 9604 is sold and referenced strictly as a research chemical. Nothing on this page is medical advice and the material is not for human consumption.

Educational and research information only. Not medical advice, not for human consumption. Values are reference figures — always verify your own math with the reconstitution calculator.

On any insulin syringe, 1 unit = 0.01 mL, so unit counts above hold across brands.