5-Amino-1MQ dosage chart
10 mg Vial
5-Amino-1MQ is dosed at 2.5 mg–5 mg daily via subcutaneous injection in educational protocols. A 10 mg vial reconstituted with bacteriostatic water yields about 5 mg/mL. This information is for research and educational use only.
- Reconstitute
- Add 2.0 mL bacteriostatic water → 5 mg/mL concentration.
- Typical daily range
- 2.5–5 mg once or twice daily (subcutaneous).
- Easy measuring
- At 5 mg/mL, 1 unit = 0.01 mL = 50 mcg (0.05 mg) on a U-100 insulin syringe.
- Storage
- Lyophilized: freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F) and use within 2–4 weeks.
Dosing schedule
| Phase | Daily Dose (mg) | Units (per injection) (mL) |
|---|---|---|
| Days 1–2 (Tolerance) | 2.5 mg (2500 mcg) | 50 units (0.50 mL) |
| Days 3+ (Standard) | 5 mg (5000 mcg) | 100 units (1.00 mL) |
| Alternative BID | 2.5 mg twice daily | 50 units (0.50 mL) × 2 |
Reconstitution steps
- 1Remove the vial from freezer storage (−20 °C / −4 °F) and allow to equilibrate at room temperature for 15–20 minutes.
- 2Draw 2.0 mL bacteriostatic water with a sterile syringe.
- 3Inject slowly down the vial wall; avoid foaming.
- 4Gently swirl/roll until dissolved—solution should be clear (do not shake).
- 5Label and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light; use within 2–4 weeks.
Important Notes
Practical considerations for consistency and safety.
- Use new sterile insulin syringes for each administration; dispose in a sharps container.
- Rotate injection sites (abdomen, thighs, upper arms) to reduce local irritation.
- Inject slowly; a mild stinging sensation may occur due to the quinolinium structure.
- Document daily dose and site rotation to maintain consistency.
- The 10 mg vial format requires multiple vials for extended protocols; plan supplies accordingly.
How This Works
5-Amino-1MQ (5-amino-1-methylquinolinium) is a synthetic small molecule that selectively inhibits Nicotinamide N-methyltransferase (NNMT). NNMT is a cytosolic enzyme that methylates nicotinamide (vitamin B3) using S-adenosylmethionine (SAM) as a methyl donor. In states of obesity and metabolic dysfunction, NNMT is often overexpressed in adipose tissue, depleting nicotinamide and reducing NAD+ availability.
By inhibiting NNMT, 5-Amino-1MQ may spare nicotinamide for NAD+ synthesis via the salvage pathway, thereby activating SIRT1 (Sirtuin 1) pathways associated with mitochondrial biogenesis, fat oxidation, and metabolic flexibility. Preclinical studies indicate that NNMT knockdown or inhibition can protect against diet-induced weight gain and improve metabolic parameters without reducing food intake.
Potential Benefits & Side Effects
Observations from preclinical and early research literature.
- May support reductions in fat mass while preserving lean muscle in animal models.
- Associated with elevated NAD+ levels and SIRT1 activation in preclinical studies.
- Enhanced grip strength observed in aged mice when combined with exercise.
- Generally well tolerated; occasional reports of mild headache, transient jitteriness, or injection-site reactions.
- Long-term human safety data not established; this compound remains investigational.
Lifestyle Factors
Complementary strategies for best outcomes.
- Pair with a balanced, protein-forward diet tailored to energy needs.
- Combine resistance training and aerobic activity—preclinical data suggest synergy with exercise.
- Prioritize sleep and stress management to support metabolic adaptation.
- Consider complementary NAD+ precursors (NMN/NR) per emerging stack protocols.
Injection Technique
General subcutaneous guidance from clinical best-practice resources.
- Clean the vial stopper and skin with alcohol; allow to dry.
- Pinch a skinfold; insert the needle at 45–90° into subcutaneous tissue.
- Do not aspirate for subcutaneous injections; inject slowly and steadily.
- Rotate sites systematically (abdomen, thighs, upper arms) to avoid lipohypertrophy.
- Slow injection may minimize any stinging sensation associated with the compound.
important-note
This content is intended for therapeutic educational purposes only and does not constitute medical advice, diagnosis, or treatment.
5-Amino-1MQ dosage FAQs
How much bacteriostatic water do you use to reconstitute 5-Amino-1MQ?
Add 2.0 mL bacteriostatic water → 5 mg/mL concentration. for a 10 mg Vial vial. Add the water slowly against the inside wall of the vial and swirl — never shake — until the powder fully dissolves.
What does the 5-Amino-1MQ dosing schedule look like?
Days 1–2 (Tolerance) — 2.5 mg (2500 mcg) — 50 units (0.50 mL); Days 3+ (Standard) — 5 mg (5000 mcg) — 100 units (1.00 mL); Alternative BID — 2.5 mg twice daily — 50 units (0.50 mL) × 2. The full table above lists every step with matching syringe units.
What are the mixing steps for 5-Amino-1MQ?
Remove the vial from freezer storage (−20 °C / −4 °F) and allow to equilibrate at room temperature for 15–20 minutes. Draw 2.0 mL bacteriostatic water with a sterile syringe. Inject slowly down the vial wall; avoid foaming.
How should reconstituted 5-Amino-1MQ be stored?
Storage: Lyophilized: freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F) and use within 2–4 weeks..
How many insulin syringe units equal a 5-Amino-1MQ dose?
Units depend on the water volume you used. Divide your target dose by the vial concentration to get millilitres, then multiply by 100 — 1 unit on a U-100 insulin syringe is 0.01 mL. The Spartan Peptides reconstitution calculator does this conversion for you.
Is 5-Amino-1MQ approved for human use?
No. 5-Amino-1MQ is sold and referenced strictly as a research chemical. Nothing on this page is medical advice and the material is not for human consumption.
Educational and research information only. Not medical advice, not for human consumption. Values are reference figures — always verify your own math with the reconstitution calculator.
On any insulin syringe, 1 unit = 0.01 mL, so unit counts above hold across brands.